gantt
dateFormat YYYY-MM-DD
axisFormat %Y-Q%q
title ADGC3 Yale [RM1MH132648]
section Grant Year
Start Grant Year 3 :milestone, year3_yale, 2025-03-31, 0d
- Start Grant Year 4 :milestone, m1, 2026-03-31, 0d
+ Start Grant Year 4 :milestone, year4_yale, 2026-03-31, 0d
Start Grant Year 5 :milestone, m1, 2027-03-31, 0d
click year3_yale href "https://docs.google.com/spreadsheets/d/10xWymZXsDlDQ9uwU5YPAKwyflUMzpDS4/edit?gid=1305684354#gid=1305684354&range=J1:M25"
+ click year4_yale href "https://docs.google.com/spreadsheets/d/1fb1A3B_nKLfhJ0FTPGUB5LgZth_eqiQ4/edit?gid=702001145#gid=702001145&range=N2:Q20"
section Aim 1
Submit initial data (L0, L1) CRISPRi MEA and calcium imaging of 15 ASD genes in iGLUTs and/or iGABAs (ARID1B - SHANK3) : crit, milestone, ADGC3_N_5, 2027-03-31, 0d
Submit initial data (L0, L1) CRISPRi ECCITEseq, KOLF2.2J and CW20107 in iGLUTs (fastq), 10 capstone genes (ARID1B - SMARCC2) : crit, milestone, ADGC3_K_6, 2025-09-30, 0d
Submit initial data (L0, L1) CRISPRi organoid models ECCITEseq of 10 capstone genes (ARID1B - SMARCC2) genes in KOLF2.2J : crit, milestone, ADGC3_N_6, 2027-03-31, 0d
Submit ECCITEseq data (L0, L1) to DRACC for 29 NDD genes (ANK3 - WAC) in induced NPCs, glutamatergic neurons, and GABAergic neurons; iGLUT 3182-3-clone5 (iNPC, d7 iGLUT and d21 iGLUT) and iGABA 553-3-clone34 (d36 iGABA) : done, milestone, ADGC3_I_7, 2025-03-31, 0d
Submit L3/L4 data from iGLUT manuscript : active, milestone, ADGC3_K_7, 2025-09-30, 0d
Submit initial data (L0, L1) iGLUT 10 capstone genes (ARID1B, ASXL3, CACNA1G, CHD8, DLL1, GABRA1, KMT2C, SCN2A, SHANK3, SMARCC2). Across four donors, KOLF2.2J, CW20107, 2607, 553, ECCITEseq resolved 7,985, 3,834, 232, and 8,902 iGLUTs, expected to further increase with final alignments. QC metrics resolved good gRNA representation and high quality/glutamatergic neurons : crit, milestone, ADGC3_Y4_N_4, 2026-06-30, 0d
Submit initial data (L0, L1) CRISPRko calcium imaging of 15 ASD genes in iGLUTs (ARID1B, ASH1L, CHD8, CUL3, DYRK1A, KMT5B, POGZ, PTEN, ANK2, CACNA1G, GRIA3, GRIN2A, NRXN1, SCN2A, SHANK3) and MEA iGLUT (SCN2A) - matrix CSV file : crit, milestone, ADGC3_Y4_N_5, 2026-06-30, 0d
Submit L3/L4 data from manuscript describing capstone ECCITEseq of 10 capstone genes in KOLF2.2J : crit, milestone, ADGC3_Y4_Q_4, 2027-03-31, 0d
Submit L3/L4 data from estradiol pre-print describing calcium imaging of 15 ASD genes and MEA of 1 gene in iGLUTs : crit, milestone, ADGC3_Y4_Q_5, 2027-03-31, 0d
%% For successful screens, submit initial data (L0, L1) CRISPR amplicon-seq of 36/250/whole genome NDD genes for neurogenesis, mitochondrial function, and/or neuronal activity : crit, milestone, ADGC3_Y4_R_6, 2028-03-31, 0d
%% Submit L3/L4 data from manuscript describing phenotyping of all genes across zebrafish + hiPSCs : crit, milestone, ADGC3_Y4_R_7, 2028-03-31, 0d
section Aim 2