55d9b1b7504ff9c43ad1173ae981a27d9212d773
hiram
  Thu May 21 11:48:01 2026 -0700
cron job safe full path names to kent commands refs #31811

diff --git src/hg/utils/automation/asmHubTOGA.pl src/hg/utils/automation/asmHubTOGA.pl
index 6d3bd940f49..343661dc2f1 100755
--- src/hg/utils/automation/asmHubTOGA.pl
+++ src/hg/utils/automation/asmHubTOGA.pl
@@ -1,185 +1,185 @@
 #!/usr/bin/env perl
 
 use strict;
 use warnings;
 use FindBin qw($Bin);
 use lib "$Bin";
 use AsmHub;
 use File::Basename;
 
 my $argc = scalar(@ARGV);
 
 if ($argc != 3) {
   printf STDERR "usage: asmHubTOGA.pl asmId asmId.names.tab .../trackData/\n";
   printf STDERR "where asmId is the assembly identifier,\n";
   printf STDERR "and .../trackData/ is the path to the /trackData/ directory.\n";
   exit 255;
 }
 
 # from Perl Cookbook Recipe 2.17, print out large numbers with comma
 # delimiters:
 sub commify($) {
     my $text = reverse $_[0];
     $text =~ s/(\d\d\d)(?=\d)(?!\d*\.)/$1,/g;
     return scalar reverse $text
 }
 
 my $asmId = shift;
 my $namesFile = shift;
 my $trackDataDir = shift;
 my $TOGABbi = `ls $trackDataDir/TOGAv*/HLTOGAannotVs*.bb`;
 chomp $TOGABbi;
 my $track = "TOGAannotation";
 
 if ( ! -s $TOGABbi ) {
   printf STDERR "ERROR: can not find trackData/TOGAv*/HLTOGAannotVs*.bb file\n";
   exit 255;
 }
 
 my $gcX = substr($asmId,0,3);
 my $d0 = substr($asmId,4,3);
 my $d1 = substr($asmId,7,3);
 my $d2 = substr($asmId,10,3);
 my (undef, $acc, undef) = split('_', $asmId, 3);
 my $accession = "${gcX}_${acc}";
 
 my $togaDir = dirname($TOGABbi);
 $togaDir =~ s#.*/trackData/##;
 
 my $bbFile = basename($TOGABbi);
 
-my $totalBases = `ave -col=2 $trackDataDir/../${asmId}.chrom.sizes | grep "^total" | awk '{printf "%d", \$2}'`;
+my $totalBases = `/cluster/bin/x86_64/ave -col=2 $trackDataDir/../${asmId}.chrom.sizes | grep "^total" | awk '{printf "%d", \$2}'`;
 chomp $totalBases;
-my $itemsBases = `bigBedInfo $TOGABbi | egrep "itemCount|basesCovered" | awk '{print \$NF}' | sed -e 's/,//g;' | xargs echo`;
+my $itemsBases = `/cluster/bin/x86_64/bigBedInfo $TOGABbi | egrep "itemCount|basesCovered" | awk '{print \$NF}' | sed -e 's/,//g;' | xargs echo`;
 my ($itemCount, $basesCovered) = split('\s+', $itemsBases);
 
 my $percentCoverage = sprintf("%.2f", 100.0 * $basesCovered / $totalBases);
 $itemCount = commify($itemCount);
 $basesCovered = commify($basesCovered);
 $totalBases = commify($totalBases);
 
 my $em = "<em>";
 my $noEm = "</em>";
 my $assemblyDate = `grep -v "^#" $namesFile | cut -f9`;
 chomp $assemblyDate;
 my $ncbiAssemblyId = `grep -v "^#" $namesFile | cut -f10`;
 chomp $ncbiAssemblyId;
 my $organism = `grep -v "^#" $namesFile | cut -f5`;
 chomp $organism;
 
 my $downloadUrl="https://hgdownload.soe.ucsc.edu/hubs/$gcX/$d0/$d1/$d2/$accession/bbi/$bbFile";
 # there is a bug in the original files, should have been GalGal6
 # the symlinks in bbi have been fixed
 $downloadUrl =~ s/galGal6/GalGal6/;
 
 print <<_EOF_
 
 <h2>Description</h2>
 <p>
 <b>TOGA</b>
 (<b>T</b>ool to infer <b>O</b>rthologs from <b>G</b>enome <b>A</b>lignments)
 is a homology-based method that integrates gene annotation, inferring
 orthologs and classifying genes as intact or lost.
 </p>
 
 <p>
 This track has $itemCount items in the track, covering $basesCovered bases
 in the sequence which is % $percentCoverage of the total sequence of size
 $totalBases nucleotides.
 </p>
 
 <h2>Methods</h2>
 <p>
 As input, <b>TOGA</b> uses a gene annotation of a reference species
 (human/hg38 for mammals, chicken/galGal6 for birds) and
 a whole genome alignment between the reference and query genome.
 </p>
 <p>
 <b>TOGA</b> implements a novel paradigm that relies on alignments of intronic
 and intergenic regions and uses machine learning to accurately distinguish
 orthologs from paralogs or processed pseudogenes.
 </p>
 <p>
 To annotate genes,
 <a href='https://academic.oup.com/bioinformatics/article/33/24/3985/4095639'
 target=blank>CESAR 2.0</a>
 is used to determine the positions and boundaries of coding exons of a
 reference transcript in the orthologous genomic locus in the query species.
 </p>
 
 <h2>Display Conventions and Configuration</h2>
 <p>
 Each annotated transcript is shown in a color-coded classification as
 <ul>
 <li><span style='display:inline-block; width:40px; height:15px; background-color:blue;'>&nbsp;</span>
     <span style='color:blue'>"intact"</span>: middle 80% of the CDS
     (coding sequence) is present and exhibits no gene-inactivating mutation.
     These transcripts likely encode functional proteins.</li>
 <li><span style='display:inline-block; width:40px; height:15px; background-color:lightblue;'>&nbsp;</span>
     <span style='color:#7193a0'>"partially intact"</span>: 50% of the CDS
      is present in the query and the middle 80% of the CDS exhibits no
      inactivating mutation. These transcripts may also encode functional
      proteins, but the evidence is weaker as parts of the CDS are missing,
      often due to assembly gaps.</li>
 <li><span style='display:inline-block; width:40px; height:15px; background-color:grey;'>&nbsp;</span>
     <span style='color:grey'>"missing"</span>: &lt;50% of the CDS is present
      in the query and the middle 80% of the CDS exhibits no inactivating
      mutation.</li>
 <li><span style='display:inline-block; width:40px; height:15px; background-color:orange;'>&nbsp;</span>
     <span style='color:orange'>"uncertain loss"</span>: there is 1
      inactivating mutation in the middle 80% of the CDS, but evidence is not
      strong enough to classify the transcript as lost. These transcripts may
      or may not encode a functional protein.</li>
 <li><span style='display:inline-block; width:40px; height:15px; background-color:red;'>&nbsp;</span>
     <span style='color:red'>"lost"</span>: typically several inactivating
      mutations are present, thus there is strong evidence that the transcript
      is unlikely to encode a functional protein.</li>
 </ul>
 </p>
 <p>
 Clicking on a transcript provides additional information about the orthology
 classification, inactivating mutations, the protein sequence and protein/exon
 alignments.
 </p>
 
 <H2>Data Access</H2>
 <p>
 The data for this track is available from the <em>bigBed</em> file format
 with the command line access tool <em>bigBedToBed</em> available from
 the utilities download directory
 <a href='http://hgdownload.soe.ucsc.edu/admin/exe/linux_x86_64/'
 target=_blank>hgdownload.soe.ucsc.edu/admin/exe/linux_x86_64</a>.
 </p>
 
 <p>
 To extract from the <em>bigBed</em> file:
 <pre>
   bigBedToBed "$downloadUrl" togaData.bed
 </pre>
 with the result in the <em>togaData.bed</em> file.
 </p>
 
 <h2>Credits</h2>
 <p>
 This data was prepared by the <a href='https://tbg.senckenberg.de/hillerlab/'
 target=_blank>Michael Hiller Lab</a>
 </p>
 
 <h2>References</h2>
 <p>
 The <b>TOGA</b> software is available from
 <a href='https://github.com/hillerlab/TOGA'
 target=_blank>github.com/hillerlab/TOGA</a>
 </p>
 
 <p>
 Kirilenko BM, Munegowda C, Osipova E, Jebb D, Sharma V, Blumer M, Morales AE,
 Ahmed AW, Kontopoulos DG, Hilgers L, Lindblad-Toh K, Karlsson EK,
 Zoonomia Consortium, Hiller M.
 <a href="https://www.science.org/doi/10.1126/science.abn3107"
 target="_blank">Integrating gene annotation with orthology inference at
 scale</a>. <em>Science</em>, 380(6643), eabn3107, 2023
 </p>
 
 _EOF_
    ;