9f9a96ab068a76799311c09a2813b0b28f8e7712
lrnassar
  Mon Jul 13 12:52:41 2026 -0700
Order EVE heatmap amino acid rows by class to match the MaveDB track. refs #37763

Change the heatmap row order from alphabetical to physicochemical-amino-acid-class order
(A V L I M F Y W R H K D E S T N Q G C P), matching the MaveDB Experiments heatmap so the
tracks can be compared row-for-row. Rebuilds eve.bb; updates the makedoc, the AutoSql row
label comment, and the description page.

diff --git src/hg/makeDb/scripts/eve/vcfToEveHeatmap.py src/hg/makeDb/scripts/eve/vcfToEveHeatmap.py
index a1422a78ef3..ced9835d73d 100644
--- src/hg/makeDb/scripts/eve/vcfToEveHeatmap.py
+++ src/hg/makeDb/scripts/eve/vcfToEveHeatmap.py
@@ -4,31 +4,31 @@
 Usage: vcfToEveHeatmap.py <vcf_dir> <output_bed>
 
 Each *_HUMAN.vcf in vcf_dir produces one heatmap BED12+ row.
   Columns = protein positions sorted by ascending genomic coordinate.
   Rows    = 20 standard amino acids (A C D E F G H I K L M N P Q R S T V W Y).
   Wildtype cell at each column is left empty.
   Colors  = blue (EVE=0, benign) -> white (EVE=0.5, uncertain) -> red (EVE=1, pathogenic).
   Mouseover includes wildtype, variant, EVE score, and Class25 classification.
 """
 
 import sys
 import os
 import re
 import glob
 
-STANDARD_AAS  = list('ACDEFGHIKLMNPQRSTVWY')   # 20 standard AAs, alphabetical
+STANDARD_AAS  = list('AVLIMFYWRHKDESTNQGCP')   # 20 standard AAs, by class, to match MaveDB
 COLOR_BOUNDS  = "0,0.5,1"
 COLOR_VALUES  = "#2166ac,#f7f7f7,#d6604d"
 LEGEND        = "EVE score: 0=benign (blue) 0.5=uncertain (white) 1=pathogenic (red)"
 
 RE_PROT_MUT = re.compile(r'^([A-Z0-9]+)_([A-Z])(\d+)([A-Z])$')
 
 
 def add_chr_prefix(chrom):
     """Convert bare chromosome names to UCSC style (1 -> chr1, MT -> chrM)."""
     if chrom.startswith('chr'):
         return chrom
     if chrom == 'MT':
         return 'chrM'
     return 'chr' + chrom