Unlike per-gene scores, popEVE is calibrated across the whole proteome, so cells are colored on a single global gradient keyed to the raw popEVE score (lower, more negative scores are more deleterious). The color is interpolated between the five anchors below: the published severe and moderate thresholds are fixed anchors, and the extremes saturate at the 0.5th and 99.5th percentiles of the proteome-wide score distribution.
| Color | popEVE score | Interpretation | |
|---|---|---|---|
| - | ≤ −5.74 | +≤ −6.04 | Most deleterious (color saturates here) |
| ≈ −5.056 | Severe threshold: high-confidence deleterious (99.99% likelihood of falling in the more deleterious distribution) | ||
| ≈ −4.617 | Moderate threshold | ||
| ≈ −3.5 | Near the proteome-wide median | ||
| - | ≥ −2.29 | +≥ −2.41 | Most tolerated (color saturates here) |
Note: popEVE ranks deleteriousness to organismal fitness, weighted toward severe, often early-onset phenotypes, rather than classic clinical pathogenicity. Some well-known disease genes whose variants act mainly through loss of function or cause adult-onset conditions (for example BRCA1) may therefore show few or no cells in the severe range.
Hovering over a cell shows a summary of that substitution and the scores behind it, for example:
G1042→A