550a0e2666bcce27181a0cb0eab32ed261e444f6 mspeir Thu Aug 27 14:56:42 2026 -0700 singleCellSignalsPeaks: color the Cell class facet checkboxes, refs #37820, refs #37914 The faceted UI already draws a color swatch beside each checkbox of any facet named in a colorSettingsUrl JSON, and the track already colors its subtracks by broad cell class from celltype-palette.tsv. Publish that palette so the selector shows the same colors: copySingleCellSignalsPeaksFiles.py now writes <bed>/singleCellSignalsPeaks_colors.json alongside the facet metadata, and the composite header names it. Rendered from the one shared palette, so a class is the same color in the checkbox list, in the drawn tracks, and on both assemblies. Co-Authored-By: Claude Opus 5 (1M context) <noreply@anthropic.com> diff --git src/hg/makeDb/scripts/singleCellSignalsPeaks/makeSingleCellSignalsPeaksRa.py src/hg/makeDb/scripts/singleCellSignalsPeaks/makeSingleCellSignalsPeaksRa.py index bbb36c5f61c..cf33e174d10 100755 --- src/hg/makeDb/scripts/singleCellSignalsPeaks/makeSingleCellSignalsPeaksRa.py +++ src/hg/makeDb/scripts/singleCellSignalsPeaks/makeSingleCellSignalsPeaksRa.py @@ -1,235 +1,240 @@ #!/usr/bin/env python3 """ Regenerate the native singleCellSignalsPeaks trackDb .ra for a genome assembly from the Cell Browser all-tracks-hub build (Redmine #37820 for hg38, #37914 for mm10). The native "singleCellSignalsPeaks" faceted composite is the Genome Browser version of the hub's main signal-&-peaks composite for that assembly (cellBrowserHg38 for hg38, cellBrowserMm10 for mm10). This script takes that composite's stanzas from the hub build and rewrites them into a native track: - the composite is renamed cellBrowser<Asm> -> singleCellSignalsPeaks - each subtrack bigDataUrl is repointed to the local /gbdb copy - subtrack colors / labels / types are carried through unchanged (so the harmonized cell-type labels and any per-track colors come along for free) The data files themselves are copied into /hive/data/genomes/<asm>/bed/singleCellSignalsPeaks/<served-relpath> (see copySingleCellSignalsPeaksFiles.py) and served via the /gbdb/<asm>/bbi/singleCellSignalsPeaks symlink; this script only (re)writes the trackDb .ra. Usage: makeSingleCellSignalsPeaksRa.py [--assembly hg38|mm10] [--stanzas STANZAS] [--meta META] [--no-meta-check] [--out OUT] """ import re, os, sys, argparse from urllib.parse import urlparse # Where the hub build writes its stanzas and metadata -- its OUTPUT dir, not its code. # The build itself lives in the cellBrowser repo (ucsc/allTracksHub), since it builds the # whole Cell Browser super hub and not just this track: # https://github.com/ucscGenomeBrowser/cellBrowser/tree/develop/ucsc/allTracksHub # Its output dir is set there by CBHUB_OUT; keep this default in step with it. HUB_BUILD = os.environ.get( "HUB_BUILD", "/hive/data/inside/cells/all-tracks-hub-build") TRACK = "singleCellSignalsPeaks" GROUP = "regulation" # ATAC-seq signal/peaks live with the ENCODE # regulatory tracks, not under singleCell ORG = {"hg38": "human", "mm10": "mouse"} # trackDb organism subdir per assembly def main(): ap = argparse.ArgumentParser() ap.add_argument("--assembly", default="hg38", choices=sorted(ORG)) ap.add_argument("--stanzas", help="hub stanza file (default: <build>/stanzas/<asm>.trackDb.txt)") ap.add_argument("--meta", help="facet metadata TSV to check the .ra against (default: the " "meta/<asm>.metadata.tsv of the build --stanzas came from)") ap.add_argument("--no-meta-check", action="store_true", help="skip the .ra-vs-metadata 1:1 check. Only for when the metadata " "genuinely does not exist; it is the check that catches a " "partly-written stanza file.") ap.add_argument("--out") args = ap.parse_args() asm = args.assembly hub_composite = "cellBrowser" + asm.capitalize() # cellBrowserHg38 / cellBrowserMm10 gbdb = "/gbdb/%s/bbi/%s" % (asm, TRACK) stanzas = args.stanzas or os.path.join(HUB_BUILD, "stanzas/%s.trackDb.txt" % asm) # Locate the rest of the build relative to the stanza file rather than off HUB_BUILD, # so that --stanzas on its own moves the whole script to another build. Pointing only # the stanzas at a second build used to check them against the default build's # metadata and abort on a mismatch that was not really there. # Layout: <build>/stanzas/<asm>.trackDb.txt and <build>/meta/<asm>.metadata.tsv build = os.path.dirname(os.path.dirname(os.path.abspath(stanzas))) meta = args.meta or os.path.join(build, "meta", "%s.metadata.tsv" % asm) out = args.out or os.path.join( os.path.dirname(os.path.abspath(__file__)), "../../trackDb/%s/%s/%s.ra" % (ORG[asm], asm, TRACK)) header = "\n".join([ "track " + TRACK, "compositeTrack faceted", "group " + GROUP, "visibility hide", "type bigBed 3", "shortLabel Single-cell ATAC-seq", "longLabel Single-cell ATAC-seq Peaks and Signals for UCSC Cell Browser datasets", "metaDataUrl %s/%s_metadata.tsv" % (gbdb, TRACK), + # Cell class -> color for the facet swatches in the selector, so a class reads the + # same in the checkbox list as in the drawn tracks (both come from + # celltype-crosswalks/celltype-palette.tsv). Written by + # copySingleCellSignalsPeaksFiles.py alongside the facet metadata. + "colorSettingsUrl %s/%s_colors.json" % (gbdb, TRACK), "primaryKey Track", "subtrackUrls Dataset=https://cells.ucsc.edu/?ds=$$", "defaultSortField Cell_class", "maxCheckboxes 200", # One scale across every selected subtrack, so two tracks drawn at the same locus # can be compared directly; with per-track autoScale, tracks whose values differ # by orders of magnitude both drew full height. It must sit on the composite, not # the children: hgTracks groups by tdb->parent (wigTrack.c setMinMax), and a # per-subtrack setting would override this one. Limits are taken from the data in # the current window, not genome-wide, so an outlying region elsewhere in the # genome cannot flatten the view. "autoScale group", ]) # class ordering for subtrack priority: palette line order (neurons, glia, # vascular, immune, ...) so same-class tracks group together in the display, # with the source (hub/dataset) order preserved within a class. The subtrack's # broad class is recovered from its color (palette is 1:1 class<->color). color_rank = {} # prefer the palette archived alongside this script (the copy of record, written by # build_celltype_crosswalks.py); fall back to the hub build dir _palf = os.path.join(os.path.dirname(os.path.abspath(__file__)), "celltype-crosswalks", "celltype-palette.tsv") if not os.path.isfile(_palf): _palf = os.path.join(build, "celltype-crosswalks", "celltype-palette.tsv") for _i, _l in enumerate(open(_palf)): _pp = _l.rstrip("\n").split("\t") if len(_pp) >= 2: color_rank[_pp[1]] = _i class_seq = {} # rank -> running counter within that class # a source path segment of "old" / "*.old" / "*_old" marks deprecated data # (e.g. cortex-atac/hub/interact.old/); the hub may keep it, but the native # track must not carry it. OLD_SEG = re.compile(r"(^|/)[^/]*(\.old|_old|\bold)($|/)", re.I) out_stanzas = [header] n = skipped_old = n_stanzas = n_parented = 0 bad_src = [] # source track names not in the cellBrowser<Asm>_ namespace for s in re.split(r"\n\s*\n", open(stanzas).read().strip()): # Dedent: the hub stanzas are indented to show their hierarchy, and this script # re-indents on write. Parsing them indented broke the "track " rename below # without tripping any count check -- subtracks kept their cellBrowser<Asm> names # and no longer matched their facet metadata rows. lines = [l.lstrip() for l in s.splitlines()] n_stanzas += 1 # Match on the parent's first token rather than the whole line. The exact-string # compare this replaces would have silently skipped every stanza if the hub ever # emitted "parent <composite> off" or changed its spacing, leaving a header-only # .ra and a zero exit status. parent = next((l for l in lines if l.strip().startswith("parent ")), "") ptoks = parent.split() if len(ptoks) >= 2: n_parented += 1 if len(ptoks) < 2 or ptoks[1] != hub_composite: continue bdu = next((l for l in lines if l.strip().startswith("bigDataUrl ")), "") rel_check = urlparse(bdu.split(None, 1)[1].strip()).path if bdu else "" if OLD_SEG.search(rel_check): skipped_old += 1 continue n += 1 # priority groups tracks by broad class (via color), source order within color = next((l.split(None, 1)[1].strip() for l in lines if l.strip().startswith("color ")), "") rank = color_rank.get(color, len(color_rank)) # unknown/uncolored last seq = class_seq.get(rank, 0); class_seq[rank] = seq + 1 priority = rank * 100000 + seq newl = [] for l in lines: if l.startswith("track "): # Validate the SOURCE name before slicing it. This used to chop a fixed # number of characters off whatever it was given, so a track not actually # named cellBrowser<Asm>_* came out silently mangled but well-formed # (BOGUS_allen_basal_ganglia_atac__dorsal -> ..._ia_atac__dorsal), which no # check on the output could catch. src_name = l.split(None, 1)[1] if not src_name.startswith(hub_composite + "_"): bad_src.append(src_name) continue suffix = src_name[len(hub_composite) + 1:] newl.append("track %s_%s" % (TRACK, suffix)) elif l.strip().startswith("parent ") and l.split()[1] == hub_composite: # "off" so every subtrack is unchecked by default; the user turns # on individual tracks via the faceted selector newl.append("parent " + TRACK + " off") newl.append("priority " + str(priority)) elif l.strip().startswith("bigDataUrl "): rel = urlparse(l.split(None, 1)[1].strip()).path.lstrip("/") newl.append("bigDataUrl %s/%s" % (gbdb, rel)) else: newl.append(l) out_stanzas.append("\n".join(newl)) # Every emitted subtrack must be "track <TRACK>_<non-empty suffix>". Checking only the # prefix was not enough: the rename always prepends it, so a source name that did not # start with the hub composite still passed, just with an empty suffix. if bad_src: sys.exit("ERROR: %d source track name(s) are not %s_* , e.g. %r. Renaming them " "would silently mangle the name. Has the hub stanza layout changed?" % (len(bad_src), hub_composite, bad_src[0])) _ok = re.compile(r"^track %s_\S+$" % re.escape(TRACK)) bad = [x.split("\n", 1)[0] for x in out_stanzas[1:] if not _ok.match(x.split("\n", 1)[0])] if bad: sys.exit("ERROR: %d subtrack(s) were not renamed into the %s namespace, e.g. %r. " "Has the hub stanza layout changed?" % (len(bad), TRACK, bad[0])) # Sanity checks: fail loudly rather than write a truncated .ra. A hub-format change # that stops the parent line matching would otherwise produce a header-only file and # exit 0, and the next trackDb load would quietly drop every subtrack. if n == 0: sys.exit("ERROR: no subtracks matched composite '%s' in %s " "(%d stanzas, %d with a parent line). Has the hub stanza format " "changed?" % (hub_composite, stanzas, n_stanzas, n_parented)) # The facet metadata is the parallel artifact: build_stanzas writes one row per # subtrack of this composite, so the counts must agree once the old-dir skips are # added back. A mismatch means the .ra and the metadata disagree, which shows up in # the browser as subtracks with no facet row (or facet rows with no track). # # Missing metadata is fatal, not a warning. This count is the only check that sees a # partly-written stanza file: the n == 0 check above catches losing every subtrack, # but a stanza file holding 22 of 925 subtracks passes it and writes a .ra that is # 903 tracks short. Warning and continuing put that hole straight back. if args.no_meta_check: sys.stderr.write("WARNING: --no-meta-check given; the .ra was not checked against " "%s. A short stanza file would not have been noticed.\n" % meta) elif not os.path.isfile(meta): sys.exit("ERROR: no facet metadata at %s, so the .ra cannot be checked against it. " "Pass --meta to point at the right file, or --no-meta-check to skip the " "check on purpose." % meta) else: with open(meta) as fh: meta_rows = sum(1 for _ in fh) - 1 # minus the header if meta_rows != n + skipped_old: sys.exit("ERROR: %s has %d rows but %d subtracks were kept (+%d old-dir " "skipped); the .ra and the facet metadata must match 1:1" % (meta, meta_rows, n, skipped_old)) # Indent subtracks under the composite, as the trackDb .ra files in the tree do # (chainNet, encode3): the container sits flush left and each level below it is # indented one step, with every line of the stanza moving together. def indent(stanza, width=4): lines = stanza.split("\n") if not any(l.startswith("parent ") for l in lines): return stanza pad = " " * width return "\n".join(pad + l if l.strip() else l for l in lines) with open(os.path.abspath(out), "w") as fh: fh.write("\n\n".join(indent(s) for s in out_stanzas) + "\n") print("wrote %s: %d subtracks (assembly=%s, composite=%s, group=%s; skipped %d old-dir)" % ( out, n, asm, hub_composite, GROUP, skipped_old)) if __name__ == "__main__": main()