31b7d4342181171f8b8271e5ed5b5cdaa22feeb6 max Wed Apr 1 07:30:30 2026 -0700 fixing up regfunc docs and tdb diff --git src/hg/makeDb/trackDb/human/hg38/regfunc.html src/hg/makeDb/trackDb/human/hg38/regfunc.html index 94f0ba80ca3..940a9f0bb03 100644 --- src/hg/makeDb/trackDb/human/hg38/regfunc.html +++ src/hg/makeDb/trackDb/human/hg38/regfunc.html @@ -1,62 +1,65 @@ <h2>Description</h2> <p> -Massively Parallel Reporter Assays (MPRAs) are high-throughput experimental methods -that test thousands of DNA sequences or genetic variants for their effects on gene -regulation. They work by linking candidate regulatory sequences to reporter genes -and measuring transcriptional output using sequencing. +Regulatory functional assays are methods that directly test whether specific +DNA sequences control gene expression - such as by acting as enhancers, +promoters, silencers, or other regulatory elements — by measuring their effect +on transcription in cells. Among these, Massively Parallel Reporter Assays +(MPRAs) are high-throughput experimental methods that test thousands of DNA +sequences or genetic variants for their effects on gene +regulation by measuring transcriptional output using sequencing. </p> <p> This track collection brings together results from two MPRA databases, one for the complete sequence fragments, one for the variants in selected fragments: </p> <ul> <li><b><a href="hgTrackUi?g=mprabase">MPRA Base</a></b> — 41,275 experimentally tested cis-regulatory elements from 51 MPRA, STARR-seq, and related reporter assay experiments, curated in the MPRA Base database (<a href="https://pubmed.ncbi.nlm.nih.gov/38045264/" target="_blank">Zhao et al., 2023</a>). </li> <li><b><a href="hgTrackUi?g=mpraVarDb">MPRAVarDB</a></b> — 242,818 variants from 18 MPRA studies, tested for effects on transcriptional regulatory activity across over 30 cell lines and 30 human diseases and traits (<a href="https://pubmed.ncbi.nlm.nih.gov/38617248/" target="_blank">Wang et al., 2024</a>). </li> </ul> <h2>Data Access</h2> <p> See the individual subtrack documentation pages linked above for detailed information on how to download and intersect the annotations. </p> <h2>Credits</h2> <p> Thanks to Tao Wang and colleagues at the University of Florida for <a href="https://mpravardb.rc.ufl.edu/" target="_blank">MPRAVarDB</a>, and to Varda Singhal and the <a href="https://pharm.ucsf.edu/ahituv" target="_blank">Ahituv Lab</a> at the University of California San Francisco for <a href="http://mprabase.ucsf.edu/app/mprabase" target="_blank">MPRA Base</a>. </p> <h2>References</h2> <p> Wang T, Matreyek KA, Yang X. <a href="https://pubmed.ncbi.nlm.nih.gov/38617248/" target="_blank"> MPRAVarDB: an online database and web server for exploring regulatory effects of genetic variants using MPRA data</a>. <em>Bioinformatics</em>. 2024 Apr 15;40(4):btae201. PMID: <a href="https://pubmed.ncbi.nlm.nih.gov/38617248/" target="_blank">38617248</a>; PMC: <a href="https://pmc.ncbi.nlm.nih.gov/articles/PMC11014600/" target="_blank">PMC11014600</a> </p> <p> Zhao J, Baltoumas FA, Konnaris MA, Mouratidis I, Liu Z, Sims J, Agarwal V, Pavlopoulos GA, Georgakopoulos-Soares I, Ahituv N. <a href="https://doi.org/10.1101/2023.11.19.567742" target="_blank"> MPRAbase: A Massively Parallel Reporter Assay Database</a>. <em>bioRxiv</em>. 2023 Nov 22;. PMID: <a href="https://www.ncbi.nlm.nih.gov/pubmed/38045264" target="_blank">38045264</a>; PMC: <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690217/" target="_blank">PMC10690217</a> </p>