2d2fec4fac516db6c64f0ae9580a44bffabb7687 brianlee Fri Mar 7 15:16:03 2025 -0800 Adding ADGC5 Scripps to Henry Gantt SSPsyGene Timelines Chart diff --git src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html index 566fe3f87bf..4228c0d5030 100755 --- src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html +++ src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html @@ -217,17 +217,58 @@ Perform pilot 10 gene electrophysiology screen : IVt12, 2024-04-01,52w Submit 5 TB of optical electrophysiology data : crit, IVt13, after IVt12, 3w Submit 5 TB of optical electrophysiology data : crit, IVt14, 2026-03-15,3w Submit 5 TB of optical electrophysiology data : crit, IVt15, 2027-03-15,3w section Aim 3 Perform census-seq screen of 40 line village RNA-seq : IVt16, 2024-04-01,52w Submit census-seq RNA-seq profiles : crit, IVt16b, after IVt16, 3w Submit 2 TB of morphology imaging : crit, IVt17, 2026-03-15,3w Submit 2 TB of optical ephys : crit, IVt18, 2026-03-15,3w Submit 2 TB of morphology imaging : crit, IVt19, 2027-03-15,3w Submit 2 TB of optical ephys : crit, IVt20, 2027-03-15,3w </pre> </div> + </div> + <!-- Start of ADGC5 Henry Gantt Chart--> + <div class="cirm-page-title back-light-blue"><h5 class="cirm-page-title-text">ADGC5 Scripps timeline</h5></div> + <div class="row"> + <div class="col-md-12"> + <pre class="mermaid"> + gantt + dateFormat YYYY-MM-DD + axisFormat %Y-Q%q + title ADGC5 Scripps + + section Grant Year + Start Grant Year 2 :milestone, m1, 2024-03-31, 0d + Start Grant Year 3 :milestone, m1, 2025-03-31, 0d + Start Grant Year 4 :milestone, m1, 2026-03-31, 0d + Start Grant Year 5 :milestone, m1, 2027-04-01, 0d + + section Aim 1 + Refine AAV-based Perturb-seq, test v1 and v2, assess data quality, finalize method : Vt1, 2024-09-01,2025-04-01 + Design gRNAs and generate vectors for Perturb-seq : Vt2, 2024-09-01,2025-04-01 + Choose single cell technology; test, validate with finalized Perturb-seq method : Vt3, 2024-09-01,2025-04-01 + Test gRNA efficacy in vitro; nominate final perturbation vectors to be used : Vt4, 2024-09-01,2025-04-01 + Discuss within and across ADGC groups to choose genes to target among list of 259 genes; finalize gene selection for this project : Vt5, 2024-09-01,2025-04-01 + + Generate multiomics data for 50 genes in 1 brain region at 1 time point : V_Y2t1, 2025-04-01,52w + Submit L0/L1 data to DRACC (Restricted) ~175 FASTQ for ~75TB overall : crit, V_Y2t2, 2025-04-01,52w + + section Aim 2 + Refine brain clearing experiments (1) test CUBIC method and assess data quality : Vt6, 2024-09-01,2025-04-01 + Refine brain clearing experiments (2) test LifeCanvas (CLARITY) method and assess data quality : Vt7, 2024-09-01,2025-04-01 + Establish data analysis pipeline through semi-automatic image tracing and segmentation : Vt8, 2024-09-01,2025-04-01 + Establish approaches to integrate single-cell omic data with spatial morphometric data : Vt9, 2024-09-01,2025-04-01 + Select first 5 ASD risk genes to be included in the study : Vt10, 2024-09-01,2025-04-01 + + Test gRNA efficacy in vitro, nominate final vectors, collect brain samples : V_Y2t3, 2025-04-01,52w + Implement functional pipeline for analyzing Perturb-seq multiomics data : V_Y2t4,2025-04-01,52w + Develop methods for GRN analysis of multiomics data : V_Y2t5, 2025-04-01,52w + Identify datasets to be used to build GRNs from developing human brains : V_Y2t6, 2025-04-01,52w + Generate raw TIFF format and 3D swc files for 100 neurons on 3 genes : V_Y2t7, 2025-04-01,52w + </pre> + </div> </div> <!--#include virtual="${ROOT}inc/footer.html" -->