8f63f65d53f80dc30cbcff4ee0906581ea4192cd brianlee Tue Aug 19 08:31:47 2025 -0700 Making SSPsyGene UCLA Aim2 milestones more readable, removed empty verbiage diff --git src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html index 2ccad65f180..f094573cedb 100755 --- src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html +++ src/hg/cirm/gateway/htdocs/sspsygeneTimeline.html @@ -46,45 +46,45 @@ Share 20 BatchA gene edited iPSC lines (10 NPD genes x 2 iPSC backgrounds) : t8, after t4, 26w Share 180 Batch B+C gene edited iPSC lines (90 NPD genes x 2 iPSC backgrounds) : t10, after t8, 39w Troubleshoot and perform 2nd attempt of difficult KO genes : t11, after t10, 13w section AIM 2 Quantify proliferation rates of NPCs from 10 Capstone iPSC clonal lines : t5, after t1, 26w Quantify proliferation rates of NPCs from all iPSC clonal lines from all batches : t9, after t4, 52w Bulk RNA-seq profiles (4 replicates per gene x (10 NPD iPSC lines + 2 controls) x 1 background (KOLF2.2J) iPSC background lines; fastq)) : t20, after t2, 52w Submit data from first capstone experiment (KOLF2.2J background) :crit, t22, after t20, 13w Bulk RNA-seq profiles ((4 replicates per gene x (10 NPD iPSC lines + 2 controls) x 1 background (CIRM line) iPSC background lines; fastq)) : t21, after t3, 39w Submit data from first capstone experiment (CIRM background) :crit, t23, after t21, 13w Begin villages : t24, 2024-07-01,13w Complete villages : t25, after t24,13w Perform StimScope Ephys Experiments for all lines : t32, 2025-10-01,30w Submit data on RNA-seq profiles from Batch B and C :crit, t33, 2025-10-01,13w - ADGC1_I_8 Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from Capstone Lines (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_I_8, 2025-03-31, 0d -ADGC1_JK_8 Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch B (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_JK_8, 2025-10-01, 0d -ADGC1_LM_8 Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch C (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_LM_8, 2026-04-01, 0d -ADGC1_NO_8 Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch D (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_NO_8, 2026-10-01, 0d -ADGC1_P_8 Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch E (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_P_8, 2026-12-31, 0d -ADGC1_Q_8 Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch F (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_Q_8, 2027-04-01, 0d + Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from Capstone Lines (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_I_8, 2025-03-31, 0d + Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch B (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_JK_8, 2025-10-01, 0d + Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch C (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_LM_8, 2026-04-01, 0d + Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch D (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_NO_8, 2026-10-01, 0d + Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch E (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_P_8, 2026-12-31, 0d + Quantify proliferation rates/IF/morphology of IPSC/NPC/Neurons from batch F (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_Q_8, 2027-04-01, 0d ADGC1_LM_111213 Submit L0-L1 from Batch B, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_LM_111213, 2026-04-01, 0d -ADGC1_NO_111213 Submit L0-L1 from Batch C, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_NO_111213, 2026-10-01, 0d -ADGC1_P_111213 Submit L0-L1 from Batch D, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_P_111213, 2026-12-31, 0d -ADGC1_Q_111213 Submit L0-L1 from Batch E-G, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_Q_111213, 2027-04-01, 0d -ADGC1_JK_14 Complete villages (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) : crit, milestone, ADGC1_JK_14, 2025-10-01, 0d -ADGC1_LM_14 Village Analysis (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) : crit, milestone, ADGC1_LM_14, 2026-04-01, 0d -ADGC1_K_15 Calcium Imaging Capstone Analysis (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) : crit, milestone, ADGC1_K_15, 2025-09-30, 0d -ADGC1_M_15 Calcium Imaging analysis excitatory/inhibitory neurons - Capstone Analysis (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) (Submit L0-L1 to DRACC on a rolling basis once completed during this timeframe) : crit, milestone, ADGC1_M_15, 2026-04-01, 0d - ADGC1_NOPQ_15 Calcium Imaging Experiments and analysis of future batches (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) (Submit L0-L4 to DRACC on a rolling basis once completed during this timeframe) : crit, milestone, ADGC1_NOPQ_15, 2027-04-01, 0d + Submit L0-L1 from Batch C, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_NO_111213, 2026-10-01, 0d + Submit L0-L1 from Batch D, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_P_111213, 2026-12-31, 0d + Submit L0-L1 from Batch E-G, producing bulk RNA-seq profiles (3 technical replicates, 4 biological replicates per gene x (20 NPD iPSC lines + 2 controls) x 2 backgrounds (KOLF2.2J/CIRM) iPSC background lines; fastq)) : crit, milestone, ADGC1_Q_111213, 2027-04-01, 0d + Complete villages - submit L0-L1 to DRACC : crit, milestone, ADGC1_JK_14, 2025-10-01, 0d + Village Analysis - submit L0-L1 to DRACC : crit, milestone, ADGC1_LM_14, 2026-04-01, 0d + Calcium Imaging Capstone Analysis (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_K_15, 2025-09-30, 0d + Calcium Imaging analysis excitatory/inhibitory neurons - Capstone Analysis (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) : crit, milestone, ADGC1_M_15, 2026-04-01, 0d + Calcium Imaging Experiments and analysis of future batches (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; tif format) (Submit L0-L4 to DRACC on a rolling basis once completed during this timeframe) : crit, milestone, ADGC1_NOPQ_15, 2027-04-01, 0d section AIM 3 Begin organoids first 20 : t26, 2024-07-01,13w Prepare samples for RNAseq : t27, after t26,13w Perform RNAseq : t28, after t27,13w Begin RNAseq Analysis : t29, after t28,13w Prepare samples for CODEX: t30, after t27,13w Perform CODEX: t31, after t30,13w Submit first Spatial Imaging data: crit, t32, after t31,13w Perform codex Capstone Lines (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; qptiff format) : crit, milestone, ADGC1_J_161718, 2025-07-01, 0d Spatial Proteomics Analysis Capstone Lines : crit, milestone, ADGC1_K_161718, 2025-09-30, 0d Perform codex Batch B (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; qptiff format) : crit, milestone, ADGC1_L_161718, 2025-12-31, 0d Spatial Proteomics Analysis Batch B : crit, milestone, ADGC1_M_161718, 2026-04-01, 0d Perform codex Batch C (10 Genes, 20 NPD iPSC lines + 2 control lines) x 2 iPSC background lines; 4 well replicates/line; qptiff format) : crit, milestone, ADGC1_N_161718, 2027-04-01, 0d Spatial Proteomics Analysis Batch C : crit, milestone, ADGC1_O_161718, 2027-04-01, 0d