6cd7003d439b355fbb5d61f2c1b80785e85c4e0e gperez2 Wed Jun 18 11:39:37 2025 -0700 Ferret musFur1 to galGal6/mm10 lastz/chain/net run for user, refs #35916 diff --git src/hg/makeDb/doc/galGal6/lastzRuns.txt src/hg/makeDb/doc/galGal6/lastzRuns.txt index 0eda517f825..ff056f563a8 100644 --- src/hg/makeDb/doc/galGal6/lastzRuns.txt +++ src/hg/makeDb/doc/galGal6/lastzRuns.txt @@ -4127,15 +4127,96 @@ sed -e 's/^/ # /;' fb.GCF_016699485.2.chainSynGalGal6Link.txt # 1023011548 bases of 1053332251 (97.121%) in intersection \ time (~/kent/src/hg/utils/automation/doRecipBest.pl -trackHub -load -workhorse=hgwdev -buildDir=`pwd` \ \ -target2bit="/hive/data/genomes/asmHubs/GCF/016/699/485/GCF_016699485.2/GCF_016699485.2.2bit" \ -targetSizes="/hive/data/genomes/asmHubs/GCF/016/699/485/GCF_016699485.2/GCF_016699485.2.chrom.sizes.txt" \ GCF_016699485.2 galGal6) > rbest.log 2>&1 grep -w real rbest.log | sed -e 's/^/ # /;' # real 32m40.423s sed -e 's/^/ # /;' fb.GCF_016699485.2.chainRBest.GalGal6.txt # 1017361581 bases of 1053332251 (96.585%) in intersection ############################################################################## +# LASTZ Chicken GalGal6 vs. Ferret MusFur1 +# (DONE - 2025-06-16 - Gerardo) + + mkdir /hive/data/genomes/galGal6/bed/lastzMusFur1.2025-06-16 + cd /hive/data/genomes/galGal6/bed/lastzMusFur1.2025-06-16 + + printf '# Ferret MusFur1 vs. Chicken GalGal6 +BLASTZ=/cluster/bin/penn/lastz-distrib-1.04.03/bin/lastz + +# TARGET: Chicken galGal6 +SEQ1_DIR=/hive/data/genomes/galGal6/galGal6.2bit +SEQ1_LEN=/hive/data/genomes/galGal6/chrom.sizes +SEQ1_CHUNK=20000000 +SEQ1_LAP=10000 +SEQ1_LIMIT=40 + +# QUERY: Ferret musFur1 +SEQ2_DIR=/hive/data/genomes/musFur1/musFur1.2bit +SEQ2_LEN=/hive/data/genomes/musFur1/chrom.sizes +SEQ2_CHUNK=20000000 +SEQ2_LAP=0 +SEQ2_LIMIT=100 + +BASE=/hive/data/genomes/galGal6/bed/lastzMusFur1.2025-06-16 +TMPDIR=/dev/shm + +' > DEF + + time (~/kent/src/hg/utils/automation/doBlastzChainNet.pl -verbose=2 `pwd`/DEF -syntenicNet \ + -workhorse=hgwdev -smallClusterHub=hgwdev -fileServer=hgwdev -bigClusterHub=hgwdev \ + -chainMinScore=5000 -chainLinearGap=loose) > do.log 2>&1 + grep -w real do.log | sed -e 's/^/ # /;' + # real 211m51.107s + + sed -e 's/^/ # /;' fb.galGal6.chainMusFur1Link.txt + # 65632006 bases of 1055588482 (6.218%) in intersection + sed -e 's/^/ # /;' fb.galGal6.chainSynMusFur1Link.txt + # 56367160 bases of 1055588482 (5.340%) in intersection + + time (~/kent/src/hg/utils/automation/doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` \ + \ + \ + galGal6 musFur1) > rbest.log 2>&1 + + grep -w real rbest.log | sed -e 's/^/ # /;' + # real 15m23.187s + + sed -e 's/^/ # /;' fb.galGal6.chainRBest.MusFur1.txt + # 61859234 bases of 1055588482 (5.860%) in intersection + + ### and for the swap + + cd /hive/data/genomes/musFur1/bed/blastz.galGal6.swap + + time (~/kent/src/hg/utils/automation/doBlastzChainNet.pl -swap -verbose=2 \ + /hive/data/genomes/galGal6/bed/lastzMusFur1.2025-06-16/DEF -swapDir=`pwd` \ + -syntenicNet -workhorse=hgwdev -smallClusterHub=hgwdev -fileServer=hgwdev -bigClusterHub=hgwdev \ + -chainMinScore=5000 -chainLinearGap=loose) > swap.log 2>&1 + + grep -w real swap.log | sed -e 's/^/ # /;' + # real 4m33.592s + + sed -e 's/^/ # /;' fb.musFur1.chainGalGal6Link.txt + # 70918404 bases of 2277906570 (3.113%) in intersection + sed -e 's/^/ # /;' fb.musFur1.chainSynGalGal6Link.txt + # 56401202 bases of 2277906570 (2.476%) in intersection +\ time (~/kent/src/hg/utils/automation/doRecipBest.pl -load -workhorse=hgwdev -buildDir=`pwd` \ + \ + \ + musFur1 galGal6) > rbest.log 2>&1 + + grep -w real rbest.log | sed -e 's/^/ # /;' + # real 17m4.241s + + sed -e 's/^/ # /;' fb.musFur1.chainRBest.GalGal6.txt + # 61922846 bases of 2277906570 (2.718%) in intersection + +real 248m52.641s +user 0m1.043s +sys 0m0.649s +##############################################################################