c6b77b666eeacc402d28f9d9585458e7960d70a5 lrnassar Wed Jan 14 17:32:42 2026 -0800 Capitalizing and fixing the referenced gene models to reference the correct mouse release vs. the human GENCODE release on both. Refs #36908 diff --git src/hg/makeDb/trackDb/mouse/mm10/clsLongReadRna.html src/hg/makeDb/trackDb/mouse/mm10/clsLongReadRna.html index 8f75d75869d..a562e3c750c 100644 --- src/hg/makeDb/trackDb/mouse/mm10/clsLongReadRna.html +++ src/hg/makeDb/trackDb/mouse/mm10/clsLongReadRna.html @@ -27,35 +27,35 @@ Views:

Model Color Coding

Model annotations are color-coded based on their incorporation into GENCODE VM36 and the assigned GENCODE VM36 BioType. Note that VM36 is not on the mm10 assembly.

Methods

This project, led by the GENCODE consortium, employed the Capture Long-read Sequencing (CLS) protocol to enrich transcripts from targeted genomic regions. It used a large capture array with orthologous probes in human and mouse genomes, targeting non-GENCODE lncRNA annotations and regions suspected of unannotated transcription. CapTrap-Seq, a cDNA library preparation protocol, was used to enrich for full-length RNA molecules (5′ to 3′).

Matched adult and embryonic tissues from human and mouse were selected to maximize transcriptome complexity. Libraries were sequenced pre- and post-capture using PacBio and Oxford Nanopore Technologies (ONT) long-read platforms, as well as short-read technologies.

Transcript isoform models were built from reads using the LyRic analysis software. These were merged using intron chains, with transcription start and end sites anchored using CAGE and poly(A) data.