bbabbd5d2566d47d923d51dbe350634783455999 mspeir Sun Oct 26 12:14:52 2025 -0700 change soe to gi, refs #35031 diff --git src/hg/htdocs/goldenPath/help/assemblyHubHelp.html src/hg/htdocs/goldenPath/help/assemblyHubHelp.html index 1dbe7943eaf..68c8e91cd4b 100755 --- src/hg/htdocs/goldenPath/help/assemblyHubHelp.html +++ src/hg/htdocs/goldenPath/help/assemblyHubHelp.html @@ -8,31 +8,31 @@

Assembly Hub User Guide

Overview

An Assembly Data Hub is a set of Internet-accessible data files that define the reference sequence to be used for a browser instance, as well as all the data files that define the annotation for that sequence. Assembly Data Hubs allow researchers to use the UCSC Genome Browser to view their own sequences with associated annotation, without the requirement that UCSC support a browser on that sequence.

Note: if you are working with a genome that has already been submitted to the NCBI Assembly system, it may already be available in the UCSC Genome Browser. -Please check the GenArk Assembly Hub collection +Please check the GenArk Assembly Hub collection to see if your genome of interest is already available. If it is not listed there, you can use the UCSC Assembly Request page to request that the genome assembly be added.

Contents

Web Server
Assembly Hub Components

Note: it is strongly recommended that each genome stanza includes defaultPos, scientificName, organism, description, so that the hub loads with meaningful defaults and can be more easily searched from the Gateway page.

2bit File

The .2bit file is constructed from the FASTA sequence for the assembly using the faToTwoBit kent program (available from the -downloads page).

+downloads page).

Example:

 faToTwoBit ricCom1.fa ricCom1.2bit
 

Use twoBitInfo to verify sequences and create a chrom.sizes file, which is not used in the hub itself but is helpful for constructing big* files:

 twoBitInfo ricCom1.2bit stdout | sort -k2rn > ricCom1.chrom.sizes
 

The .2bit file can also be hosted at a URL:

 twoBitInfo -udcDir=https://genome.ucsc.edu/goldenPath/help/examples/hubExamples/hubPlants/cshl2013/ricCom1/ricCom1.2bit stdout | sort -k2nr > ricCom1.chrom.sizes